An ideal experimental setup to acquire significant fluorescence images of works of art should allow: - to radiometrically calibrate the fluorescence emission, to characterize spectrally and colorimetrically the radiation emitted; - to classify the painting fluorescence emission image, after the proper calibration, using a database of the spectral and colorimetric characteristics of the fluorescence emitted by materials (dyes, pigments, varnishes, binding media), commonly used by artists and restorers, replacing with quantitative data, the current empirical association of perceived fluorescence colors with materials; - to perform the acquisition even in presence of stray light; - to minimize the UV radiation dosage employed while acquiring the fluorescence images, since UV radiation causes chemical alterations and artificial ageing of the materials composing the painting surface; - to reproduce the fluorescence images according to the CIE standards.